Pregunta:
Example flow cytometry method: DNA and protein in yeast cells
Autor: Kate JarosRespuesta:
1. grow yeast and fix cells 2. incubate in RNAase solution for 1 hour 3. Stain with dyes. Fluorescein isothiocyanate for protein, propidium iodide for DNA 4. Wash cells and suspend in buffer 5. Test in flow cytometer
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Autor
Kate Jaros