How is principle of CAR-T cell therapy in cancer?

— Blood is collected from the cancer patient by leukapheresis — T cells are selected by purification on Ab coated beads. Most suitable are T-memory stem cells — T cells are transfected virally or non-virally with a CAR molecule and expanded in vitro — Modified T-cells are reinfused to the patient with or without combined chemotherapy. T cells bind to antigen-expressing tumor cells and kill them — Advantage: — Ag binding by Ab-binding site, not TCR: no need for APC, no MHC restriction — Higher number of specific T cells — Intracellular signaling domains overcome suppressive microenvironment