What types of processing are needed?

Goal: deliver the correct dose of key cells and minimize reactions 1. ABO incompatibility: RBC depletion of bone marrow necessary for major mismatches A&B blood cannot be transfused into non-compatible types Plasma depletion can be done for minor mismatches PBSC generally have low RBC content Small amounts of incompatible RBCs can be tolerated 2. In Vitro T-cell Depletion: CD34+ selection :Removal of all cells except CD34+ cells CD3+ depletion, CD3+/CD19+ depletion