(Mechanisms facilitating DISTRIBUTION TO A TARGET) - Accumulation in Cell Organelles 1 __ accumulate in lysosomes as well as mitochondria. 2 Lysosomal accumulation OCCURS BY __, that is, DIFFUSION OF THE AMINE in UNPROTONATED FORM into the ACIDIC INTERIOR of the organelle, where the amine is protonated, PREVENTING its EFFLUX, so that it IMPAIRS PHOSPHOLIPID DEGRADATION. 3 Mitochondrial accumulation takes place __. The AMINE is PROTONATED in the INTERMEMBRANE SPACE and then sucked into the matrix space by the STRONG NEGATIVE POTENTIAL (–220 mV), where it may IMPAIR β OXIDATION and OXIDATIVE PHOSPHORYLATION.