What are different types of gating?

1. flow stability gating: elimination of cells that are affected by clogging or other instrument-related issues. increases sensitivty of the experiment. 2. pulse geometry gating: elimation of doublet cells/clumped cells (as indicated by their disproportionate width). 3. forward and side scatter gating: identifiy the cells based on their size (FSC) and complexity (SSC) and remove any debris or cells that are not of interest (often cells in the bottom left of the graph). 4. subsetting gating: elimation of many unwanted cells, maintaining only cells that express the marker of interest. viability dyes are often used to eliminate dead cells. 5. backgating: done at the end of gating analysis, it allows for the visualisation of cells in a certain gate at a higher level and it determines whether any cells are being missed by previous gating strategies.