What data is generated after flow cytometry?

A voltage over time curve is generated. the area of the curve is an indicator of the signal that is generated. the height of the curve is an indicator of the intensity of the signal and the width of the curve tells you something about the time it takes for the cell to pass through the laser (bigger width, more time). the data can be plotted linearly or logarithmically. the linear scale is often used when you analyse FSC and SSC and the log scale is used to analyse fluorescent scatter. when the data are log transformed, the resolution is increased and different peaks can be detected more easily. data can be presented in a histogram, dot plots (each dot represents a cell), contour plots and density plots (higher densities will have a different colour).