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MBS1002 Biomedical Approaches

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Pregunta:

What are the pros and cons of qPCR?

Autor: Mirte Schattorie



Respuesta:

Pros: faster than PCR, real-time monitoring, efficiency of reaction can be precisely calculated, quantitative analysis, high sensitivity and specificity, widely applicable, high throughput. cons: more expensive than PCR, quality and quantity can affect results, can produce false negatives if target sequence is only present at low concentrations, no novel information, limited to detection of DNA and RNA.


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