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MBS1002 Biomedical Approaches

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Pregunta:

What are different protein labelling techniques?

Autor: Mirte Schattorie



Respuesta:

Stable isotope labelling metabolic (SILAC): label introduced in first stage of analysis, very accurate labelling. chemical labelling: an isotope coded affinity tag (ICAT) is added to cysteines. isobaric peptide tagging: labelling is done after protein extraction and denaturation (all tags have identical mass).


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