THEORY Fluorescence spectrometry/FLUOROMETRY / spectrofluorometry

An emission phenomenon e- of a molecule prefer to reside at the lowest vibrational level of the ground electronic state e- promoted to excited electronic energy state reside in one of these vibrational energy levels Time of residence is short (10-8 to 10-4 s) Reverted back to ground state: collision deactivation and radiative process (fluorescence and phosphorescence)