Molecular cloning of GFP

In 1992, Douglas Prasher reports the cloning of the gene encoding avGFP into E.coli. Quickly realises the protein can work in many species and is independent of co-factors. Prasher suggests using GFP as a reporter molecule in diverse cells, for both gene expression studies and protein fusion. Before leaving, passed his cDNA clone of avGFP to Martin Chalfie and Roger Tsien