Diagnosis of TB

1. Smear test- microscopic visualization of acid-fast bacilli in the sputum. 2. X-ray of the chest to detect patches on the lungs. 3. Physiology- night sweats, temperature, persistent cough, weight loss, fatigue. 4. Cultivation sputum using highly growth sensitive systems such as the MGIT. 5. Tuberculin skin test (Mantoux test) for hypersensitivity to antigens of Mtb. 6. IFN-gamma release assay. 7. T-SPOT.TB assay for activated TB-specific effector T cells (TB ELISpot). 8. Detection of Mtb specific antigens in the blood. 9. Detection of specific genes using qPCR GeneXpert targets Mtb using rpoB, also will detect rifampicin resistance.