An assay for wound healing in vitro

Chemotaxis is important for multiple responses in the human body including wound healing and for inflammatory response. In this in vitro wound healing assay a confluent layer of cells in a petri dish are disturbed. Cells migrate into the space. This movement, which mimics wound healing is disrupted if the cells express dominant negative versions of either Rac, Cdc42 or Rho GTPases.