Dam1/DASH complex (pt.2)

The Dam1/DASH complex can bind to the + TIPs of both polymersing and depolymerising microtubules. In this experiment Rhodamine-coupled tubulin is nucleated in vitro and Alexa-488 coupled Dam1 complex is added. At time = 0, Dam1 ring complexes are seen decorating the length of the microtubule. As the microtubule depolymerises the signal at the depolymersing plus end increases as Dam1 ring complex accumulate there. This suggests it can act as a ring in vitro.