Mechanism of translocation

EF-G/GTP binds to the pre-translocation ribosome at a site including L7/L12, L11 and the sarcin/ricin loop of 23S rRNA. The tRNA-like domain interacts with the 30S subunit close to the partial A site. GTP hydrolysis induces a conformational change in EF-G, forcing its arm deeper into the 30S subunit, which forces the peptidyl tRNA from the A site into the P site, carrying the mRNA and deacylated tRNA with it. RESULT:-ribosome moves along the mRNA by length of one codon