23S rRNA in the peptidyl transferase reaction

N3 of A2486 accepts a proton from the amino group of the aminoacyl tRNA in the A site. This enhances the negative charge of the amino group, allowing it to attack the bond between the peptide and tRNA in the P site. The N3 of A2486 H-bonds to the oxyanion in the tetrahedral intermediate stabilising it and accelerating the reaction. The 3’-OH of the tRNA in the P site accepts the proton from A2486, completing the reaction. – Ribozyme activity