Control of Initiation of Replication (E.coli)

OriC has been characterised. DNA digested with a restriction enzyme and then ligated into a plasmid lacking an origin of replication. Origin actin region is 245 bps long and contains: 1. 14 copes of GATC 2. 4/5 copies of a 9 bp seq in the right hand 2/3 oriC 3. three copies of AT-rich 13 bp seq in the left hand 1/3 of oriC.