Investigating the effects of temperature on yeast respiration (anaerobic respiration) (1)

- Put known vol. + conc. of substrate solution in test tube - Add known vol. of buffer solution - Place test tube in water bath - Set 1 of the temps. being investigated - Leave for 10 mins - Add known mass of dried yeast, stir - After yest has dissolved, add liquid paraffin - This will stop O2 getting in, forces yeast to respire anaerobically - Put bung, with tube attached to a gas syringe, in the top of tube