Procedure

1. Patients’ serum/plasma added to each well in the screen cell set along with LISS. 2. Incubate at 37C to allows any antibodies present to react with the antigens (Sensitization) 3. Washing of the well to remove unbound antibodies 4. Adding indicator cells (coated with Anti-IgG, to allow reaction to occur) 5. Centrifugation for several minutes