1. What group of ABO Discrepancy is this test helpful? Why this phenomenon does cause discrepant ABO Typing?

- It is useful in Group Iv Discrepancy. Unexpected ABO isoagglutinins may cause discrepancy in the patient’s serum when it react at room temperature with the corresponding antigen present on the reagent cells. A2 and A2B individuals are examples of this type of ABO discrepancy which can produce naturally occurring anti-A1, or A1 and A1B, individuals who may produce naturally occurring anti-H. The patient’s RBCs can be tested with Dolichos biflorus to confirm the presence of the ABO subgroup. Dolichos biflorus will agglutinate cells of the A1 but not the A2 phenotype