MAKING D-CONTROL TUBE:

A. Label a tube with patient initials and “D ctrl” below initials. b. Place 1 drop of patient RBC suspension in the tube c. Add 1 drop of Rh control reagent to the labeled tube and mix d. Centrifuge for 15-20 seconds e. Shake gently to resuspend f. AB Positive - If tube is negative, it confirms the patient is AB Pos; however, if agglutination is observed, further testing is required before a blood type can be determined. STOP HERE for AB Pos. g. D Negative – continue with Weak D test (Indirect antiglobulin testing)