PROCEDURE: A. SALIVA COLLECTION AND PREPARATION:

1. Collect about 2 to 3 mL of saliva in a test tube. 2. Centrifuge at 900 to 1000 rpm for 8 to 10 minutes. 3. Transfer supernatant to a clean test tube, and place stoppered tube in a boiling water bath for 10 minutes. This inactivates enzymes that might otherwise destroy blood group substances. 4. Recentrifuged at 900 to 1000 g for 8 to 10 minutes. 5. Collect clear supernatant into a clean tube.