Newer probes used in nucleic acid detection of chlamydia trachomatis and N. gonorrhoeae use MSU may obviate the need of direct sampling in uncomplicated cases and in evaluation of sexual abuse. some methods of NAD include

1) pcr (can deteect 10-100 EBs) 99/99. rapid and relaible diagnosis. 2)lcr 3) strand displacemnt amplification (SDA) 4) HYbrid capture system(HCS) 5) transcription mediated amplification (TMA) or RNA 6) specific probes for DNA