Whole Genome Sequencing Methods

1. DNA extraction (culture bacterial cells, lyse cells to release DNA, purify DNA) 2. DNA cutting (enzymatically cut the DNA with restriction endonucleases, producing short fragments. can use mechanical shearing) 3. preparation of DNA library (DNA fragments placed in PCR thermocycler, PCR amplifies DNA fragments to form copies) 4. DNA sequencing (DNA library placed into sequencer, nuclotides of each DNA fragment are read, yielding millions of DNA reads) 5. DNA sequence analysis (computer-aided analysis of millions of DNA reads, program puts reads together in the correct order)