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Pregunta:

PCR proces

Autor: Kate Jaros



Respuesta:

Each 3 step process --> one cycle 1. denature target dsDNA to ssDNA 2. anneal (join) primers 3. extend primers by enzymatic addition of nucleotides - each cycle doubles the amount of DNA target - typical amplification is 20-40 cycles - results in a million-fold amplification of DNA


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each 3 step process --> one cycle
1. denature target dsDNA to ssDNA
2. anneal (join) primers
3. extend primers by enzymatic addition of nucleotides

- each cycle doubles the amount of DNA target
- typical amplification is 20-40 cycles
- results in a million-fold amplification of DNA
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