The slide is then placed under microscope and observed for parasitic eggs. o A drop of toluene or xylol may be placed between the tape and the slide to clear the preparation. o The specimen should be collected for 3 consecutive days at early in the morning before the patient has taken a bath or before the patient has washed the perineum; can also be obtained late at night when patient have already slept for several hours o At least 4 to 6 consecutive negative tapes are required to rule out the infection.