Sanger Method (7)

Used to determine DNA sequence 4 sets of PCR with normal nucleotides and 1 dideoxynucleotide is used (ddATP, ddTTP, ddCTP, ddGTP) whenever the DDON is used the sequence is terminated bec PCR = 1 billion copies so every possible fragment will be made for a base When the fragments are separated by GEL ELECTROPHORESIS base sequence is determined by ordering the fragments according to their lengths fragments fluorescently tagged for automated sequencing