Explain 4 mechanisms of an allosteric regulation of Hb binding to O2 that facilitate O2 transport?

2,3-Bisphosphoglycerate Another heterotropic allosteric regulator of O2 Binding to Hemoglobin • 1- Negative heterotropic regulator of O2 binding. • 2- Present at mM concentrations in erythrocytes – Produced from an intermediate in glycolysis • 3- Small negatively charged molecule, binds to the positively charged central cavity of Hb • 4- Stabilizes the T states The Ph difference between lungs and tissue increases efficiency of the O2 transport(Bohr effect). undergoing a transition from a low-affinity state (the T state) to a high- affinity state (the R state) as more O2 molecules are bound.