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Lehninger ch3 bioschemistry

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Pregunta:

Types of chromatography and what are their principles

Autor: Shay 1vcc



Respuesta:

-column chromatography which takes advantage of differences in protein charge, size, binding affinity, and other properties -Ion-exchange chromatography exploits differ- ences in the sign and magnitude of the net electric charge of proteins at a given pH -cation-exchange chromatography the solid matrix has negatively charged groups. In the mobile phase, proteins with a net positive charge migrate through the matrix more slowly than those with a net negative charge, -Size-exclusion chromatography, separates proteins according to size. In this method, large proteins emerge from the column sooner than small ones -Affinity chromatography is based on binding affinity


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