| transgenic organism | when a gene from one organism is inserted into the chromosomes of another organism
-new genes passed onto the next generation |
| key breakthroughs leading to recomnbinant DNA | restriction enzymes (cut)
DNA ligase (DNA joining enzyme) (glue) |
| restriction enzymes -overveiw + cut patterns | Cut DNA across double helix
each enzyme has a specific recognition site
cuts hydrogen bonds
blunt ends, sticky ends |
| Creating recombinant DNA steps: | 1. gene of interest isolated and cut w/ restriction enzyme
2. bacteria plasmid or phage vector also cut
3. plasmid and gene mixed in solution
4. DNA ligase added to join sticky ends of plasmid and gene
5. new recombinant vector inserted into the host cell (bacterium, fungus, or animal cell)
6. host replicates, replicating plasmid as well. |
| rDNA uses | artificially manufacture proteins ie insulin, gh, fsh
manufacture vaccines ie hep b vaccine
gene therapy |
