| What does monoclonal antibodies mean | mono-1 type of b cell
clonal- divided by mitosis
antibody - produces them |
| using small mammals to produce antibodies | 1. inject rabbit
2.rabbit produces primary immune response
3.rabbit produces b cells
4. b cell collected and stimulated to produce antibodies in petri dish |
| using mab for cancer treatment | mab is a protein
specific tertiary structure
has specifc binding site shape
complimentary to antigen on cancer cells
mab binds forming antibody antigen complex
drug on mab kills cancer cells |
| what can they be used to detect | antigens on pathogens
drug testing
pregnancy testing |
| What does Elisa mean | Enzyme linked immunosorbent assay |
| Trigger words for HIV TESTING | ANTIGEN
ANTIBODY
WASH
ANTIBODY WITH ENZYME
WASH
SUBSTATE COLOUR |
| Antiobodies in medical treatment | antibodies target cancer cells by binding to specific antigens
deliver drugs to cancer cells to treat it. |
| Elisa test full | antibody binds to complimentary antigen
wash to remove unbound antigens
second antibody with enzyme attatched is added
second antibody attaches to first antibody
wash to remove unbound antibodies again
substrate is added to - binds to enzyme active site - complex - colour change |
