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level: allen exercises

Questions and Answers List

level questions: allen exercises

Question	Answer
the most likely reason for the development of resistance against pesticides in insect damaging a crop is	random mutations
the conventional method for naming the restriction enzymes is followed. in case of Eco. R 1, the R indicates	strain
after completing transformation experiment involving the coding sequence of enzyme beta-galactosidase, the re-combinant colonies should	not give blue colour
which of the following method can be used for making the bacterial cell competent	treating with specific conc. of divalent cation (Ca2+) heat shock
during heat shock to the bacterium, the temperature used for giving thermal shock is	43 degree C
uring isolation of DNA, addition of which of the following causes precipitation of purified DNA	chilled ethanol
if we ligate a foreign DNA at the Bam H1 site of tetracycline resistance gene in pBR322 the recombinant plasmid will	show ampicillin resistance only
which can be used as vector for transfer of DNA segment	plasmid and bacteriophage
in order to insert alien DNA at the site tetracycline resistance gene in PBR 322 the restriction endonuclease used can be	sal-I
PCR is use to	amplify a small amount of DNA
what is the criterion for DNA fragments movement on agarose gel during gel electrophoresis	the smaller the fragment size, the father it moves
which of the following is not a feature of the plasmids	single-stranded
the taq polymerase enzyme is obtained from	thermus aquaticus
which of the following is a restriction enonuclease	HIND II
stirred-tank bioreactors have been designed for	availability of oxygen throughout the process
which of the following restriction enzymes producs blunt ends	Eco RV
the DNA fragments sepearated on an agarose gel can be visualized after staining with	ethidium bromide
the process of separation and purification of expressed protien before marketing is called	downstream processing